RESUMO
Os carrapatos são ectoparasitas obrigatórios e vetores de agentes patogênicos como bactérias, parasitos e vírus. O presente estudo teve como objetivo identificar a presença de bactérias dos gêneros Rickettsia, Ehrlichia e Anaplasma em carrapatos de vida livre coletados em cinco áreas, localizadas nas mesorregiões Sul Fluminense e Metropolitana do Rio de Janeiro. Foram coletados 9.353 carrapatos distribuídos em 372 pools. A análise dos dados pelo método PCR-RFLP indicou que as bandas verificadas na análise eram de Rickettsia bellii em 0,25% dos carrapatos coletados de duas áreas. A pesquisa molecular para os gêneros Ehrlichia e Anaplasma revelou resultados negativos para ambos os gêneros. A presença de genoma de R. bellii em carrapatos sugere a importância da execução de novas pesquisas com o intuito de entender a epidemiologia dos agentes estudados na região.
Ticks are obligate ectoparasites and vectors of several bacterial, parasitic and viral pathogens. This study aimed to identify bacteria of the Rickettsia, Ehrlichia and Anaplasma genera in free-living ticks, collected in five areas located in both South Fluminense and Metropolitan mesoregions of Rio de Janeiro state. We collected a total of 9,353 ticks which were distributed in 372 pools. Data analysis using the PCR-RFLP method showed that the bands observed in the analysis are of the type Rickettsia bellii. The positivity level to R. bellii found in this study was 0.25%. The molecular search for Ehrlichia and Anaplasma genera revealed negative results for both genera. The presence of Rickettsiae DNA in the Rocky Mountain Spotted Fever group in ticks suggests the importance of undertaking new research in order to understand the epidemiology of the agents.
Assuntos
Animais , Carrapatos , Bactérias , Epidemiologia , Ehrlichiose , Ectoparasitoses , AnaplasmoseRESUMO
The present paper describes the morphological modifications occurring during the larval development of Habronema muscae (Nematoda: Habronematidae) in Musca domestica (Diptera: Muscidae), along with the reactions caused by parasitism and the migration route of the nematodes inside the flies. Houseflies were reared on faeces of a H. muscae-infected horse, then dissected and processed by histology. The experimental part of the study was performed in 1996 in the Parasitological Experimental Station W.O. Neitz, Federal Rural University of Rio de Janeiro, Seropédica, Rio de Janeiro State, Brazil. Three different larval stages of H. muscae were recovered, measured and described. The encapsulation of larval nematodes was found in the third larval instar (L3) of M. domestica and cryptocephalic pupa. The mature capsules were observed in dipteran L3, pupae and mainly adults. In 1day-old or more M. domestica adults an active rupturing of capsules by H. muscae L3 and the migration to the head through the circulatory system and insect hemocoel were observed. Infective H. muscae L3s remained exclusively in the head of adult 5days-old or more M. domestica.
